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Copyright© Bentham Science Publishers; For any queries, please email at epub@benthamscience.net.OBJECTIVE Early exposure to general anesthesia in children might be a potentially high-risk factor for learning and behavioral disorders. The mechanism of neurotoxicity induced by general anesthesia was not defined. miR-496 could regulate cerebral injury, while the roles of miR-496 in neurotoxicity were not elucidated. Therefore, we aimed to investigate the effects of miR-496 in neurotoxicity induced by propofol. METHODS Primary prefrontal cort