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To explore the application of serum peptidomics in the early diagnosis of pancreatic ductal adenocarcinoma (PDAC). The serum specimens from 176 patients with PDAC and 158 healthy control patients were subjected to matrix-assisted laser desorption ionization time-of-flight mass spectrometry to obtain serum peptide profiles. Next, a classification model by differentiated peptides was established and verified to distinguish the 2 groups. Finally, the peptides were identified by tandem mass spectrometry. A classification model was established by 13 pept