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This article is protected by copyright. All rights reserved.RATIONALE For pharmaceutical quality control, impurities may have unexpected pharmacological or toxicological effects that seriously impact on safety and efficacy. Arginine vasopressin (AVP) is an important cyclic peptide drug, which is mainly used for the treatment of diabetes insipidus and esophageal varices bleeding. With the improvement of analytical techniques, liquid chromatography-high resolution mass spectrometry (LC/hrMS) has become a critical technique for the chall