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To overcome this issue, we recently designed and built much-improved UV irradiation and cell harvesting devices. Using these new tools, we developed a protocol for time-resolved analyses of RBP-RNA interactions in living cells at high temporal resolution Kinetic CRoss-linking and Analysis of cDNAs (χCRAC). We recently used this technique to study the role of yeast RBPs in nutrient stress adaptation. This manuscript provides a detailed overview of the χCRAC method and presents recent results obtained with the Nrd1 RBP.A human alveolar cel

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The United States is now experiencing public health catastrophe on a scale we have not seen for more than a century. COVID-19 puts into stark relief our mutual obligations that reflect our interdependence as participants in a common society. Drawing on the work of Amartya Sen concerning famine and related challenges, I discuss the accompanying implications for social justice. Social justice in catastrophe requires strong social insurance structures and legal protections for the most vulnerable people, who would otherwise lack economic r

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In spite of many decades of intensive research on Ixodes ricinus, the castor bean tick of Europe, several important aspects of its basic biology remain elusive, such as the factors determining seasonal development, tick abundance and host specificity, and the importance of water management. Additionally, there are more recent questions about the geographical diversity of tick genotypes and phenotypes, the role of migratory birds in the ecoepidemiology of I. ricinus, the importance of protective immune responses against I. ricinus, particu

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Postprocedure sexual satisfaction scores were significantly higher than preprocedure values (8 vs 6, respectively; P=.002). In the first follow-up of a patient after the 3rd injection, a 4-mm diameter fibrotic plaque was observed on the ventral side in the middle of the penis shaft. In conclusion, our findings suggest that larger studies as well as placebo-controlled studies are needed to add PRP to the treatment protocol in ED. T Taş,Çakıroğlu, E Arda, etal. Early Clinical Results of the Tolerability, Safety, and Efficacy of Autologous

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FileMagic is a versatile and user-friendly file management software designed to enhance the way users handle a variety of file types. For users who frequently encounter compressed files, especially 7Z files, FileMagic offers a seamless, convenient way to open and manage these formats without the need for specialized knowledge or multiple programs. As is well-known, 7Z files are a popular compression format used to reduce file size, enabling speedier transfers and more economical storage. However, without , acc

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The optimal continuous peripheral nerve block (CPN technique for total hip arthroplasty (THA) that maximizes both analgesia and mobility is unknown. Continuous erector spinae plane (ESP) blocks were implemented at our institution as a replacement for fascia iliaca (FI) catheters to improve our THA clinical pathway. We designed this study to test the hypothesis that this change will increase early postoperative ambulation for elective primary THA patients. We identified all consecutive primary unilateral THA cases six months before a

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For all future ihMT studies we recommend careful positioning of the subject within the scanner.Background The Makerere University/Uganda Virus Research Institute (UVRI) Centre of Excellence for Infection Immunity Research and Training (MUII) is a collaborative programme supporting excellence in Infection and Immunity (II) research in Uganda. Set up in 2008, MUII aims to produce internationally competitive Ugandan and East African II research leaders, and develop human and infrastructural resources to support resear

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The use of zebrafish (Danio rerio) in biomedical research has expanded at a tremendous rate over the last two decades. Along with increases in laboratories using this model, we are discovering new and important diseases. We review here the important pathogens and diseases based on some 20 years of research and findings from our diagnostic service at the NIH-funded Zebrafish International Resource Center. Descriptions of the present status of biosecurity programmes and diagnostic and treatment approaches are included. The most common and

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AIMS Leucocyte-directed specialized pro-resolving mediators (SPMs) are essential for cardiac repair, and their biosynthesis coincides with the expression of pro-inflammatory mediators; however, the precise quantitation during an acute myocardial infarction (MI) event is poorly understood in race-specific and sex-specific manner. Coronary heart disease is the leading cause of death and disability in the USA. Although the prevalence of coronary heart disease is similar between Black and White patients, cardiovascular events (including MI

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Managing pain can be challenging, especially in patients with serious illnesses and a history of substance use disorders. This article discusses the challenges of addressing pain in these patients and offers perspectives regarding their clinical management. Managing pain can be challenging, especially in patients with serious illnesses and a history of substance use disorders. This article discusses the challenges of addressing pain in these patients and offers perspectives regarding their clinical management. Idiopathic pulmonary fib