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© 2020 John Wiley Sons, Ltd.Spirulina microalga (Arthrospira platensis) is an interesting phototrophic organism because its high content of nutrients including proteins, lipids, essential amino acids, antioxidants, vitamins, polysaccharides and minerals. Hydrophilic interaction liquid chromatography (HILIC) coupled to linear ion trap (LIT) and Orbitrap Fourier transform mass spectrometry (FTMS) via electrospray ionization (ESI) was employed for the separation and characterization of lipid species in A. platensis. Inositolphosphocera