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Additionally, δ13 C was not correlated with gas exchange estimates of WUEi under short- and long-term water limitations but showed a significant negative relationship with WUEplant . The observed association between the δ13 C and WUEplant suggests an intrinsic link of δ13 C with WUEi in this C4 plant and can potentially be used as a screening tool for WUEplant in Sorghum.The CRISPR/Cas9 and Cas12a (Cpf1) tools have been used on a large scale for genome editing. A new effector with a single nuclease domain, a relatively small size, low-f