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Intra-day and inter-day precisions (relative standard deviation) were 10%. Ang II, Ang III, Ang IV, and Ang (1-5) were positively correlated with ACE expression by HUVECs, while Ang I, Ang (1-7), and Ang (1-9) were negatively correlated. The nanoflow liquid chromatography-parallel reaction monitoring mass spectrometry-based methodology established in this study can evaluate the Ang peptides simultaneously in HUVEC culture supernatant. The nanoflow liquid chromatography-parallel reaction monitoring mass spectrometry-based methodology e
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