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To investigate the prevalence of Clostridium perfringens alpha toxin encoding gene and C.perfringens enterotoxin encoding gene in dogs with acute haemorrhagic diarrhoea syndrome. Retrospective study looking at the prevalence of C. perfringens alpha toxin and C. perfringens enterotoxin in the faeces of three groups of dogs - those with acute haemorrhagic diarrhoea syndrome (n=16), those with haemorrhagic diarrhoea from another cause (n=17) and those without haemorrhagic diarrhoea (n=1. Correlation between the presence of C. p