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AIMS Hemolysis of serially-collected insulin serum samples frequently causes falsely-low measured concentrations due to release of intracellular insulin degrading enzyme (IDE). We investigated if bacitracin, an in vitro IDE inhibitor, could prevent hemolysis-induced insulin degradation during insulin sensitivity testing. MATERIALS AND METHODS Blood samples were collected from adults undergoing serial sampling for insulin sensitivity. A dose-finding study measured insulin from experimentally-hemolyzed samples containing five bacitracin co
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