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Electron bifurcation uses free energy from exergonic redox reactions to power endergonic reactions. β-FAD of the electron transfer flavoprotein (EtfA from the anaerobic bacterium Acidaminococcus fermentans bifurcates the electrons of NADH, sending one to the low potential ferredoxin and the other to the high potential α-FAD semiquinone (α-FAD·-). The resultant α-FAD hydroquinone (α-FADH-) transfers one electron further to butyryl-CoA dehydrogenase (Bcd); two such transfers enable Bcd to reduce crotonyl-CoA to butyryl-CoA. To get insight
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