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Trichophyton rubrum (Tr) is the main aetiological agent of human dermatophytosis, being isolated from the environment and keratinised tissues. In the environment, Tr can interact with other organisms, such as free-living amoebas (FLA), which can act as an alternative host system to study the interaction between microbes and phagocytic cells. To characterise the Acanthamoeba castellanii (ALX)-Tr interaction. Interaction was characterised in three conditions trophozoites (PYG), late (PYG/NES) and early (NES) encystation stimulus, evaluati