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To the best of our knowledge, the present study was pioneer in reporting PE MPs-induced behavioral toxicity in representatives of amphibian groups. The activation rate of oxygen by zero-valent iron (Fe°) was very low. In this study, ethylenediaminetetraacetic acid (EDTA), oxalate (Ox), and phosphate ions (Na2HPO4) were used to enhance the oxygen activation by Fe° for sulfamethazine (SMT) removal. The addition of these ligands could significantly enhance the SMT degradation. SMT removal was improved from 10.5 % in the Fe° system (360 min
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