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international populations had little influence on protein intake. This trial was registered at the ISRCTN registry as ISRCTN46157745 (https//www.isrctn.com/ISRCTN4615774). Copyright © The Author(s) on behalf of the American Society for Nutrition 2020.RNA polymerases initiate transcription at DNA sequences called promoters. In bacteria, the best conserved promoter feature is the AT-rich -10 element; a sequence essential for DNA unwinding. Further elements, and gene regulatory proteins, are needed to recruit RNA polymerase to the -10 sequ