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Identification of the specific biomarkers is of great importance to enrich and expand the gonocytes or spermatogonial stem cells (SSCs) in livestock. The glial cell line-derived neurotrophic factor (GDNF) family receptor alpha-1 (GFRα-1) is a conserved marker of the gonocytes/SSCs in multiple species including rodents, primates and human; however, its expression in bovine gonocytes/SSCs is debated. Recently, we and other teams clearly demonstrated the expression of GFRα-1 in bovine gonocytes/SSCs. This is useful for bovine gonocytes/SSCs
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