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Accordingly, we unearthed that our protospacer adjacent motif (PAM)-free CRISPR/Cas12a-assisted RPA assay, which just moderately hampered RPA at its ideal CRISPR/Cas12a focus, outperformed its alternatives in assay design, signal, susceptibility, and rate. We additionally found that a fresh commercial Cas12a effector could also drive our PAM-free CRISPR/Cas12a-assisted RPA assay and lower its cost, though simultaneously decreasing its sign. Our study plus the new i
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