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We investigated isoprene (ISO) emission and gas exchange in leaves from different positions along the vertical canopy profile of poplar saplings (Populus euramericana cv. '74/76'. For a growing season, plants were subjected to four N treatments, control (NC, no N addition), low N (LN, 50 kg N ha-1year-1), middle N (MN, 100 kg N ha-1year-1), high N (HN, 200 kg N ha-1year-1) and three O3 treatments (CF, charcoal-filtered ambient air; NF, non-filtered ambient air; NF + O3, NF + 40 ppb O3). Our results showed the effects of O3 and/or N on s
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