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Macrophages were utilized to evaluate differential immune responses due to variations in the AuNP-BC. Results AuNPs form distinct BCs based on physicochemical properties and the surrounding environment, with the obese BC containing more proteins and fewer lipids than the healthy BC. Differential macrophage inflammatory responses were observed based on AuNP properties and BC composition. Discussion and Conclusion Overall, these findings demonstrate that AuNP size and coating, as well as physiological environment, influence the protein a