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Numerous natural basic products (NPs) could modulate UPR and useful for healing reasons. Herein, we aimed to research the molecular procedure of Cycloart-23E-ene-3β, 25-diol (Cycloart-E25), cytotoxicity, as a NP extracted from Euphorbia macrostegia and centered on endoplasmic-reticulum stress (ERS) and UPR signaling paths. Reactive oxygen types (ROS) were probed by DCFDA fluorescence dye. Apoptosis had been assayed by annexin V/propidium iodide (PI), immunoblotting of anti- and proapop