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in situ hybridization and Western-blotting were used to detect the expression of P2X4R mRNA and protein in the DRG. These results showed that OECs had good biocompatibility with CS. Compared with the CCI, the MWT and TWL were significantly increased (P<0.05), the expression levels of P2X4R mRNA and protein in the OECs and OECs+CS group were significantly reduced (P<0.05). Compared with the OECs, the expression levels of P2X4R mRNA and protein in the OECs+CS group were significantly reduced (P<0.05), the MWT and TWL were