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d to sustainable drug release behavior, which would provide a beneficial approach for drug delivery technology. Brucellosis is a zoonotic disease and prevalent in livestock animals. The bacteria reside inside the macrophage cells of host. The WHO endorsed the combination treatment therapy for brucellosis as compared to monotherapy to avoid relapse and resistance. Therefore, we developed nanoparticles incorporating doxycycline and rifampicin in combination. The aim of the study is to develop polymeric nanoparticles incorporating doxycycline