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After each robot end tool replacement, tool center point (TCP) calibration must be performed to achieve precise control of the end tool. This process is also essential for robot-assisted puncture surgery. The purpose of this article is to solve the problems of poor accuracy stability and strong operational dependence in traditional TCP calibration methods and to propose a TCP calibration method that is more suitable for a physician. This paper designs a special binocular vision system and proposes a vision-based TCP calibration algorithm t