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The co-contamination by perfluoroalkyl acids (PFAAs) and heavy metals (HMs) is ubiquitous in the surface environment subjected to sewage irrigation and land application of sludge. However, the joint effects of HMs and PFAAs on plant roots are not well clarified. This study explored the root uptake and acropetal translocation behaviors of C2-C8 PFAAs by wheat (Triticum acstivnm L.) under the co-exposure of copper (Cu). The underlying uptake mechanisms of PFAAs were verified in a defective root system. The results showed that excessive Cu (
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