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Surface selection of PM-IRRAS was demonstrated by suppression of water and phosphate signals in buffers with monolayers of oleic acid. Phosphate signals were shown to reflect relative concentrations. Absorption peaks attributable to phospholipids were detected by PM-IRRAS on the human tear film surface and were augmented by the addition of phospholipid. The data provide strong evidence that phospholipids are present at the surface of tears. The data provide strong evidence that phospholipids are present at the surface of tears.Recent a