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The goal of this study was to investigate the status of FEN1 in Colorectal cancer (CRC) and determine the potential correlation between FEN1 expression level and clinicopathological parameters in CRC patients. Expression of FEN1 in CRC tissue on tissue microarray was detected using immunohistochemistry (IHC). The relationship between FEN1 expression status and clinicopathologic characteristics of CRC was analyzed by Chi-square test. The survival data of TCGA Colon Cancer (COAD) were obtained from ucsc xena browser (https//xenabrowser.ne