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To evaluate Ultrasound Imaging (USI) reliability for measurement of lumbar multifidus (LMF) muscle thickness and cross sectional area (CSA) at rest and during contraction in patients with unilateral lumbar disc herniation. Laboratory. Reliability Study. Thirty patients, aged 25-50 years (37.55 ± 9.55), with unilateral L4-L5 lumbar disc herniation participated in this study. Thickness and CSA of LMF were measured using B-mode ultrasound by two raters in prone position. Same day and multiple day inter-rater and same day intra-rater reliabi