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Concentrations of 62 FTS in substrate decreased substantially by 30 d, likely driven by microbial action. PFOS had notable biota-sediment accumulation factors, but still less then 1. While a no-observable-effect concentration could not generally be determined, the lowest observable effect concentration was 50 - 120 ppb. Survival was not affected. Our work demonstrates that PFAS bioaccumulation from dermal exposures and sublethal effects are dependent on species, chemical, and focal trait. This article is protected by copyright. All right