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The present study aimed to assess the therapeutic effect of chitosan nanoparticles and metronidazole against as well as evaluate the efficacy of loading metronidazole on chitosan nanoparticles. This study was carried out at medical Parasitology Department, Faculty of Medicine, Zagazig University and Theodor Bilharz Research institute (TBRI) from February 2019 to February 2020 on 45 hamsters. They were divided into 5 groups 9 hamsters each Group A non-infected hamsters, Group B infected control group, Group C, D and E infected with and t