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© 2020 American Association of Public Health Dentistry.One of the most frequently applied techniques in zebrafish (D. rerio) research is the visualisation or manipulation of specific cell populations using transgenic reporter lines. The generation of these transgenic zebrafish, displaying cell- or tissue- specific expression of frequently used fluorophores such as GFP or mCherry, is relatively easy with modern techniques. Fluorophores with different emission wavelengths and driven by different promoters can be monitored simultaneously in th