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Specificity was confirmed by the non-amplification of the sequences belonging to other species, yeasts, molds, bacteria, or human DNAs. The standard curve of the assay showed a highly significant linearity between threshold values and dilution rates (R =0.99; slope=-3.42). The applied qPCR assay facilitated the rapid and accurate identification and quantification of emerging opportunistic . Therefore, considering the promising test validation results, we succeeded to develop a rapid and accurate hydrolysis probe- based qPCR assay for the
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