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The Unified Assay is a comprehensive workflow wherein exome-enriched RNA-sequencing (RNA-Seq) assays are performed on clinical samples and reviewed by a series of advanced level machine learning-based classifiers. Gene expression signatures and uncommon and/or novel genomic activities, including fusions, mitochondrial variations, and lack of heterozygosity were evaluated making use of RNA-Seq data generated from 120,313 clinical samples across three clinical indications (thyr