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08% ± 3.55 with rMPC to 0.78% ± 2.04 with EnMPC (P less then 0.05).Carbon dots (CDs) were synthesized by a microwave-mediated method and separated by size exclusion chromatography into three different size fractions. There was no correlation of the size with photoluminescence (PL) emission wavelength, which shows that the PL mechanism is not quantum-size dependent. UV/vis absorption and diffuse reflectance spectroscopies showed that the light absorption properties as well as the band gap of the CDs changed with the size of the particle.