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It has been reported that stomatal conductance often limits the steady-state photosynthetic rate. On the other hand, the stomatal limitation of photosynthesis in fluctuating light remains largely unknown, although, in nature, light fluctuates due to the changes in sun position, cloud cover and the overshadowing canopy. In this study, we analyzed three mutant lines of Arabidopsis thaliana with increased stomatal conductance, to examine to what extent stomatal opening limits photosynthesis in fluctuating light. The slac1 (slow anion chann